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Our studies indicate that viable DPSC can be obtained from teeth stored for â‰¥72hrs, these can then be immortalized and still produce functional neurons for in vitro studies, but that constitutive hTERT immortalization is not be the best approach for long term use of patient derived DPSC for the study of disease. Immortalization significantly altered the expression of 183 genes after neuronal differentiation, 94 of which also changed during differentiation. Here we show that these changes in gene expression are concurrent with changes in protein levels of the transcriptional repressor REST/NSRF, which is known to be involved in neuronal differentiation.

We also show that differentiation of DPSC into neurons significantly alters gene expression for 1305 transcripts. We show that neurons from immortalized DPSC share morphological and electrophysiological properties with non- immortalized DPSC. We immortalized control DPSC using human telomerase reverse transcriptase (hTERT) and evaluated neuronal differentiation potential and global gene expression changes by RNA-seq. We investigated the basic transport conditions and maximum passage number for primary DPSC. To investigate the characteristics of DPSC that make them a valuable resource for translational research, we performed a set of viability, senescence, immortalization and gene expression studies on control DPSC and derived neurons. Dental pulp stem cells (DPSC) are multipotent stem cells that reside deep the pulp of shed teeth. Although several sources of stem cells are currently available, acquiring these involve invasive procedures, may be difficult or expensive to generate and are limited in number. Donaldson, Martin Bridges, Dave Reiter, Lawrence T.Ī major challenge to the study and treatment of neurogenetic syndromes is accessing live neurons for study from affected individuals. Scroggs, Reese Miranda-Carboni, Gustavo A. Urraca, Nora Memon, Rawaha El-Iyachi, Ikbale Goorha, Sarita Valdez, Colleen Tran, Quynh T.

To facilitate the future application of these cells in studies of neurological dysfunction and neuronal replacement therapies, a comprehensive.Ĭharacterization of Neurons from Immortalized Dental Pulp Stem Cells for the Study of Neurogenetic Disorders Recently, we established and phenotypically characterized an immortalized porcine olfactory bulb neuroblast cell line, OBGF400 (Uebing-Czipura et al., 2008). USDA-ARS?s Scientific Manuscript database Transcriptome profile and cytogenetic analysis of immortalized neuronally restricted progenitor cells derived from the porcine olfactory bulb

Revista para colorir evangelica download